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Features of telephone rules bargains with a few of the facets of mobile regulate mechanisms.
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0 SCRAPINGS ISOLATED CELLS FIG. 1 I . Calcium binding to Golgi membrane vesicles prepared from intestinal scraping or isolated cells of normal and vitamin D-deficient rats. Data from Walters Cf a/. (1984). 38 MILTON M. , 1984). However, Pisam and Ripoche (1976), studying isolated frog bladder epithelial cells, and Ziomek et al. (1980), studying isolated mouse intestinal epithelial cells, urged caution in defining membranes derived from isolated cells prepared from previously organized tissue.
1973) procedure through the CaCI, precipitation step, with two subsequent higher speed centrifugation precipitations of microvillus membranes. Peters (1976), using hypoosmolar Tris/EDTA and sorbitol density gradient centrifugation, isolated microvilli from the human jejunum but did not separate the membrane from the core, although he did separate microvilli from the lateralbasal membrane. In chicks, Max et al. (1978) combined the standard method for purifying nuclei with Tris disruption and glycerol density gradient centrifugation to first remove contaminating nuclei from a nuclei-microvilli precipitate and then proceeded by methods similar to those of Eichholz and Crane (1965) to separate plasmalemma from core material.
1982). These studies were based on microvillus preparations by Hopfer er al. , 1977). When studies revealed that most of the Ca2 uptake by these membrane preparations was due to the presence of nonesterified fatty acids (Walters and Weiser, 1984a,b), an explanation for the source of these fatty acids was sought. One important observation was that if isolated cells were used as the initial tissue preparation prior to homogenization, the uptake of Ca2 by membrane vesicle preparations was markedly reduced (Fig.