Cannabinoid Analysis in Physiological Fluids by Joseph A. Vinson

By Joseph A. Vinson

content material: A survey of metabolic alterations of [delta]¹-tetrahydrocannabinol / Sumner Burstein --
GLC and HPLC analyses of cannabinoids in organic fluids and functions / E.R. Garrett, A.J. Gouyette, and C.A. Hunt --
Detection and quantitation of tetrahydrocannabinol in physiological fluids / Monroe E. Wall, Dolores R. Brine, Joan T. Bursey, and David Rosenthal --
Quantitation of [delta]⁹-tetrahydrocannabinol and 11-nor-[delta]⁹-tetrahydrocannabinol-9-carboxylic acid in physique fluids via GC/CI-MS / R.L. Foltz, P.A. Clarke, B.J. Hidy, D.C.K. Lin, A.P. Graffeo, and B.A. Petersen --
development of the mass fragmentographic strategy for quantification of tetrahydrocannabinol in human blood plasma / Agneta Ohlsson, Stig Agurell, Jan-Erik Lindgren, and Kurt Leander --
A normal method of the research of cannabinoids from physiological resources / J. Rosenfeld --
Quantitation of [delta]⁹-tetrahydrocannabinol and its metabolites in human urine through chance established matching GC/MS / Donald E. eco-friendly, Fu-Chuan Chao, Kay O. Loeffler, and Saul L. Kanter --
HPLC research of [delta]⁹-tetrahydrocannabinol and metabolites in organic fluids / Seth R. Abbott, John R. Berg, Kay O. Loeffler, Saul Kanter, Leo E. Hollister, Joan Hawkins Abrams, Hugh L. Baras, and Reese T. Jones --
Radioimmunoassay of cannabinoid compounds / C.E. cook dinner --
Antisera raised opposed to tetrahydrocannabinol within the radioimmunoassay of cannabinoids / J.D. Teale, Jacqueline M. Clough, L.J. King, V. Marks, P.L. Williams, and A.C. Moffat --
HPLC analyses of [delta]⁹-tetrahydrocannabinol and 11-nor-[delta]⁹-tetrahydrocannabinol-9-carboxylic acid in human plasma / Jimmie L. Valentine, Owen H.M. Gan, H.C. Nio, and Everett D. Thompson --
Detection and quantitation of [delta]⁹-tetrahydrocannabinol in plasma through dansylation and double labeling / J.M. Scherrmann, R. Bourdon, H. Hoellinger, Nguyen-Hoang Nam, and E. Fournier.

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9 9 L Hydroxylation in vivo in other quently singly at 11 or position in positions. observed combination Oxidation to In with vitro and hydroxylation carbonyl also fre­ observed. 9 — Hydroxylation at 8 series*, 8β both 8a or 8,11-dihydroxy 1 7α, 7β or position metabolites 7,11-Dihydroxy , * Figure 1. found found as only minor only Δ -THC metabolites* frequently found in in observed. g Δ -series. , H y d r o x y l a t i o n at 1 ,2*,3 ,4' o c c a s i o n a l l y found, a l s o as Ι',ΙΙ; 2 ' , 1 1 - e t c .

2. GARRETT ET AL. GLC and 35 HPLC Analyses f a c e a f f i n i t y o f the d r u g . However, t h e c o m p e t i t i o n o f a l a r g e degree o f u n s a t u r a b l e b i n d i n g (97%) t o plasma p r o t e i n s m i n i m i z e s t h e amounts i n t h e r e d b l o o d c e l l s o f whole b l o o d , a l t h o u g h p o s s i b l y l a r g e adherence o f t h e d r u g t o t h e w a l l s o f b l o o d v e s s e l s and t o t h e s u r f a c e s o f t h e t i s s u e s must be a n t i c i p a t e d .

F o l l o w i n g a d d i t i o n o f each i n t e r n a l s t a n d a r d the plasma sample was s t i r r e d f o r 3-5 seconds i n a v o r t e x a g i t a t o r and then s u b j e c t e d t o s o n i c a t i o n (Cole-Parmer u l t r a s o n i c c l e a n e r ) f o r the same t i m e . 5% i s o a m y l a l c o ­ h o l . The t u b e s were a g i t a t e d 15 minutes each time i n a v o r t e x a g i t a t o r and the l a y e r s s e p a r a t e d by c e n t r i f u g a t i o n a f t e r each e x t r a c t i o n .

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