Drug Discovery Science and Development in a Changing Society by Gould R.F. (ed.)

By Gould R.F. (ed.)

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26 Potential of Native Sucrose-Utilizing Transglucosidases for Glucoside Synthesis . . . . . 1 A General Mechanism . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 Natural Promiscuity Is at the Heart of Large Product Diversity . . . . . . . . . . . 31 3 Engineering Novel Sucrose-Utilizing Transglucosidases . . . . . . . . . . . . . . . . . . 1 Enzyme Engineering Strategies . . . .

Denis N, Meiser A, Riese J, Weihe U (2008) Setting the right course in biobased bulk chemicals 1:22 20 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38. 39. 40. 41. 42. 43. 44. 45. 46. 47. 48. 49. 50. 51. 52. S. Peters et al. 2009. 2009) Claus P, Vogel GH (2006) Chem Ing Tech 78:991 Weidenhagen R, Wegner H (1938) Chem Ber 71:2712 Ling GV, Driessen AJ, Piet AC, Vligter JC (1970) Ind Eng Chem Prod Res Dev 9:210 Wright LW (1976) US 3 965 199 (CI America Inc) Weissermel K, Arpe H-J (2003) Industrial organic chemistry.

Polymer elongation has been shown to proceed I. Andre´ et al. 4) Amylose Osidic linkage a-1,6 (>50%) a-1,3 (>50%) a-1,4 (>50%) Alternating a-1,3/a-1,6 a-1,4 (100%) through glucosyl unit transfer at the non-reducing end of the acceptor molecules (glucose, sucrose, or other primers initially produced) [69, 70]. Reaction conditions such as substrate concentration, pH, and temperature were also shown to have an undeniable influence on the structure of products synthesized by glucansucrases, in particular the degree of branching and the polymer size [41, 71–75].

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