Fluorescence In Situ Hybridization (FISH) — Application by Nedime Serakinci, Steen Kølvraa (auth.), Thomas Liehr (eds.)

By Nedime Serakinci, Steen Kølvraa (auth.), Thomas Liehr (eds.)

This FISH software consultant offers an outline of the foundations and the elemental options of fluorescence in situ hybridization (FISH) and primed in situ hybridization (PRINS), that are effectively used to review many elements of genomic habit and adjustments. In 36 chapters, contributed via overseas specialists of their specific box, the these days a number of techniques and functions of the robust options are awarded and designated protocols are given.

Described listed here are equipment utilizing a variety of telephone kinds and tissues in addition to varied organisms, reminiscent of mammalians, bugs, vegetation and microorganisms. Multicolor FISH approaches and unique functions resembling the characterization marker chromosomes, breakpoints, cryptic aberrations, nuclear structure and epigenetic alterations, in addition to (array-based) comparative genomic hybridization stories are offered. total, the means of selection is brought for unmarried mobilephone research in human genetics, microbiology, animal and plant sciences.

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Fluorescence In Situ Hybridization (FISH) — Application Guide

This FISH software consultant offers an summary of the rules and the elemental thoughts of fluorescence in situ hybridization (FISH) and primed in situ hybridization (PRINS), that are effectively used to check many facets of genomic habit and adjustments. In 36 chapters, contributed by means of foreign specialists of their specific box, the these days a number of techniques and purposes of the strong recommendations are provided and exact protocols are given.

Additional info for Fluorescence In Situ Hybridization (FISH) — Application Guide

Example text

A balance between tissue preservation and tissue/chromosome digestion must be found. If the tissue/chromosomes are preserved too well, it the DNA probes may not be able to pass through, so that no result is obtained from FISH. In the case of too much tissue/chromosome digestion, FISH signals may still be obtained, but it may not be possible to correlate them to a specific tissue/chromosomal region. Complete loss of the tissue/chromosomes during the FISH procedure might occur. It is recommended that beginners should start with target samples that are not limited in availability.

In the case of too much tissue/chromosome digestion, FISH signals may still be obtained, but it may not be possible to correlate them to a specific tissue/chromosomal region. Complete loss of the tissue/chromosomes during the FISH procedure might occur. It is recommended that beginners should start with target samples that are not limited in availability. 2 Denaturation for FISH Denaturation times of only 2–4 min are suggested for the maintenance of available metaphase chromosomes. When working with tissues without metaphase spreads this aspect is of no significance.

Obstet Gynecol 108:612–616 Chin SF, Daigo Y, Huang HE, Iyer NG, Callagy G, Kranjac T, Gonzalez M, Sangan T, Earl H, Caldas C (2003) A simple and reliable pretreatment protocol facilitates fluorescent in situ hybridisation on tissue microarrays of paraffin wax embedded tumour samples. Mol Pathol 56:275–279 Clay H, Ramakrishnan L (2005) Multiplex fluorescent in situ hybridization in zebrafish embryos using tyramide signal amplification. Zebrafish 2:105–111 Collins AR, Oscoz AA, Brunborg G, Gaivao I, Giovannelli L, Kruszewski M, Smith CC, Stetina R (2008) The comet assay: topical issues.

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