Human Cell Culture: Volume VI: Embryonic Stem Cells by Tenneille Ludwig, James A. Thomson (auth.), John R. Masters,

By Tenneille Ludwig, James A. Thomson (auth.), John R. Masters, Bernhard O. Palsson, James A. Thomson (eds.)

If you want to develop or symbolize embryonic stem cells or convince them to distinguish right into a specific telephone variety, then this booklet includes details that's very important on your good fortune. the purpose is to supply transparent easy directions and protocols for starting to be, conserving and characterizing embryonic stem cells and info of many of the tools used to make stem cells differentiate into particular telephone kinds. The contents should be of curiosity to stem telephone biologists, tissue engineers and scientists wishing to exploit embryonic stem cells for healing reasons. every one bankruptcy has been written and edited by means of the world over revered scientists operating on the innovative of technological advancements in human embryonic stem cells.

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M. (1998) Embryonic stem cell lines derived from human blastocysts. Science, 282: 1145–1147. qxd 18/4/07 4:45 PM Page 41 CHAPTER 4 GENETIC MODIFICATION OF HUMAN EMBRYONIC STEM CELLS THOMAS P. ZWAKA Baylor College of Medicine, Center for Cell and Gene Therapy and Department of Molecular and Cellular Biology, Houston, Texas, USA 1. INTRODUCTION Embryonic stem (ES) cells are derived from an early embryonic stage during which the cellular machinery is geared toward rapid expansion and diversification, simultaneously possess both the ability for unlimited self-renewal and the potential to differentiate into derivatives of all three germ layers (Pedersen 1986).

Qxd 18/4/07 4:44 PM Page 27 CHAPTER 3 CHARACTERIZATION AND DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS ANDREW L. LASLETT1,2, ADELIA LIN3, AND MARTIN F. PERA1,3,4 1 The Australian Stem Cell Centre Department of Anatomy and Cell Biology, Monash University, Clayton, Australia 3 Monash Institute of Medical Research, Monash University, Clayton, Australia 4 Center for Stem Cell and Regenerative Medicine, Keck School of Medicine, University of Southern California 2 1. INTRODUCTION This chapter will discuss the characterization and differentiation of human embryonic stem cell (hESC) lines.

Science, 282: 1145–1147. , and Benvenisty, N. (2004) Modeling for Lesch-Nyhan disease by gene targeting in human embryonic stem cells. Stem Cells, 22: 635–641. , and Kuliev, A. (2005) Human embryonic stem cell lines with genetic disorders. Reprod. Biomed. Online, 10: 105–110. Wilmut, I. (2004) Human cells from cloned embryos in research and therapy. BMJ, 328: 415–416. P. A. (2003) Homologous recombination in human embryonic stem cells. Nat. , 21: 319–321. qxd 18/4/07 4:44 PM Page 27 CHAPTER 3 CHARACTERIZATION AND DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS ANDREW L.

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